Immunofluorescent analysis of p-Cadherin (red) in A431 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a p-Cadherin mouse monoclonal antibody, AlexaFluor 647 conjugate (Product # MA3-24000-A647), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.